Abstract
Gastric ulcers result from an imbalance between endogenous defense mechanisms and certain aggressive agents. Many drugs were used to overcome this imbalance, but few literatures made on plants. Therefore, we try to evaluate the gastroprotective efficacy of two nutritional supplements (Vanadyl sulfate and Lycopene) in comparison to Lansoprazole. Five groups of seven healthy albino male rats each were received an oral daily dose of above agents for ten days. Then 1.25 ml of 95% ethanol orally used to induce mucosal injury and animals were sacrificed 1 hour later. Glutathione and malondialdehyde were estimated. A significant elevation in glutathione level found in Vanadyl and Lycopene-received groups in comparison to lansoprazole-received group (717.13±19.47 μmol/gm wet tissue, 609.55±17.6 μmol/gm wet tissue and 512.07±25.32 μmol/gm wet tissue respectively), with a significant reduction in malondialdehyde level (10.63±0.92 nmol/gm wet tissue, 12.66±0.56 nmol/g wet tissue and 14.90±0.33 nmol/gm wet tissue respectively). This revealed gastro-protective effects of Vanadyl and Lycopene in ameliorating the oxidative cellular damage.
Highlights
We try to evaluate the gastroprotective efficacy of two nutritional supplements (Vanadyl sulfate and Lycopene) in comparison to Lansoprazole
A significant elevation in glutathione level found in Vanadyl and Lycopene-received groups in comparison to lansoprazole-received group (717.13±19.47 1⁄4mol/gm wet tissue, 609.55±17.6 1⁄4mol/gm wet tissue and 512.07±25.32 1⁄4mol/gm wet tissue respectively), with a significant reduction in malondialdehyde level (10.63±0.92 nmol/gm wet tissue, 12.66±0.56 nmol/g wet tissue and 14.90±0.33 nmol/gm wet tissue respectively)
Gastric mucosal lesion is formed when aggressive factors go beyond the cellular selfdefense mechanisms
Summary
Materials They were bought from Al-Madinah known drug bureau (lansoprazole was procured from Actavis /Barnstaple/UK, Vanadyl sulfate from Aldniuds Co./Germany, and lycopene from Liptis Pharmaceuticals /USA). They were carried out according to (1) the LD of 95% ethanol was 6 ml/kg p.o.12 (2) the safe American recommended dose of oral vanadyl sulfate is 5 mg/kg/day 13 and (3) lycopene has been given in doses as high as 2000mg/kg/day without any adverse effects[14]. We decided 56% ethanol as 8g/kg once daily[15] and 1/10th of upper doses of vanadyl sulfate and lycopene to be considered for the experiments[16]. After rinsing with cold saline, the specimen was put in[10] milliliter of potassium chloride (KCl) solution for 45 minutes to have a homogeneous solution. Mixture was cooled in an ice-containing baker for 10 minutes At this time, 5 milliliters of n-butanol: pyridine (15:1) was added. After centrifuging for 10 minutes, malondialdehyde level in each supernatant was measured using High. All tested and control drugs showed a high significant reduction in free radicals of gastric tissue extract through increasing GSH and decreasing MDA levels (Table II)
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