Evaluation of Free-Radical Scavenging and Antioxidant Activity of Fruit Extracts of Three Endemic Rhamnus Species in in vitro

Abstract

The antioxidant properties of Rhamnus petiolaris Boiss., R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel., and R. thymifolius Bornm., three endemic species occurring at altitudes of 1000 – 1200 meters on volcanic rock around Afyonkarahisar was evaluated. Antiradical activity of Rhamnus petiolaris Boiss., R. thymifolius Bornm. and R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel was found 0.47, 0.56, and 0.68 respectively. Chelation of the samples via Fe2+ ranged from 38.78 % to 54.79 %. R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel showed the highest chelation via Fe2+, which was 54.79 %. Another method to determine the antioxidant activity of the samples was elimination of H O , which appeared to be 36.12 % in Rhamnus petiolaris Boiss., 53.89 % in R. thymifolius Bornm., and 68.732 %2 in R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel. Also for this test, R. rhodopeus Velen. subsp. anatolicus (Grub.) Browicz & Ziel exhibited the highest antioxidant activity.