Abstract
ABSTRACTA method for differentiation ofCampylobacter jejuniandCampylobacter colibased on focal‐plane‐array Fourier transform infrared (FPA‐FTIR) spectroscopy was evaluated as an alternative to the current cumbersome methodology. Two types of reference data banks were constructed, one containing FTIR spectra ofC. jejuniandC. colistrains, and the other containing FTIR spectra of 11Campylobacterspecies. The FPA–FTIR method was tested by identifying 40C. jejuniand 16C. coliisolates from poultry, previously identified biochemically and by a multiplex polymerase chain reaction, through comparison of their spectra against the data banks. Employing theC. jejuniandC. colidata bank produced high sensitivity towardC. jejuni(95%) andC. coli(94%) and high overall specificity (95%). With theCampylobacterspp. data bank, the corresponding values were 82, 88 and 84%, respectively. We conclude that FPA‐FTIR spectroscopy is a valuable tool for the differentiation ofC. jejuniandC. coli,particularly whenC. jejuniandC. colidata banks are used.PRACTICAL APPLICATIONSFoodborneCampylobacterinfections are highly prevalent, and therefore proper detection and identification ofCampylobacterstrains are of paramount importance. Accurate and fast methods for the identification ofCampylobacter jejuniandCampylobacter coliare pressing needs. This study presents a rapid, accurate method for differentiation betweenC. jejuniandC. colibased on Fourier transform infrared (FTIR) spectroscopy. By employing FTIR imaging instrumentation equipped with an infrared microscope and a focal‐plane‐array detector, thousands of spectra are recorded from each isolate in the amount of time a traditional FTIR spectrometer records a single spectrum. In turn, rich biochemical characterizations of bacterial strains, often termed “whole‐organism fingerprints,” are rapidly produced. Comparison of the FTIR spectra of unknown microorganisms against spectral databases of reference strains through the use of principal component analysis allows quick and accurate identification ofC. jejuniandC. colistrains, offering an invaluable tool for food safety assurance and surveillance.
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