Abstract
Purpose: Electrochemical measurements have prompted the progress as a consequence of their affectability, cost-affectivity and comparatively short examination time. The aim of this study was the fast evaluation of the effect of chemotherapy compounds on the viability of lung cancer cells (A549) via electrochemical methods.Methods: Cyclic voltammetry (CV) was used as a primary method to distinguish between electrochemical behavior of normal and lung cancer cells. Differential pulse voltammetry (DPV) was employed as a complementary analyses method for the impact of doxorubicin (DOX) and Flavonoid modified drug (FMD) (US patent Application number: 62548886) on Lung cancer cells.Results: Only one oxidative peak, at approximately -0.15 V was detected through DPV method in cancer cell line. While a significant distinguish was not seen in CV. The current intensity (I) was decreased in cancer cells with increasing the DOX and FMD levels (t=99.027, α=0.05, P=0.0000), (t=135.513, α=0.05, P=0.0000), respectively.Conclusion: The movement of cancerous cells towards death through chemotherapy drugs such as DOX and FMD can make distinct and significant changes in the electrochemical behaviors of those cells.
Highlights
The intercellular communications, intracellular action and peripheral reactions of cell organelles are as yet not completely comprehended and ensemble averaged measurement of millions of cells together, being not able to give point by point data at single cell level
Cyclic voltammetry (CV) was used as a primary method to distinguish between electrochemical behavior of normal and lung cancer cells
The Differential pulse voltammetry (DPV) was be used to quantify the effect of DOX concentration on A549 cell line as follows: Effect of DOX = 100%× (Ap control – Ap DOX)/ Ap Control being Ap DOX and Ap control the peak areas of DPV obtained for A549 cells treated with and without DOX, respectively
Summary
The intercellular communications, intracellular action and peripheral reactions of cell organelles are as yet not completely comprehended and ensemble averaged measurement of millions of cells together, being not able to give point by point data at single cell level. DPV give data about the chemical form of target analytes, as complexation and oxidation status This technology has likewise been generally used for the electrochemical examination of proteins and cells.[3,4] Lung cancer is one of the main sources of cancer related mortality around the world,[5] being the second most ordinarily recognized malignancy in men and women, that involved approximately 224,390 new cases and 158,080 deaths in 2016 as indicated by the American Cancer Society.[6] Because of that it is an important point to make an early diagnostics of lung cancer and to evaluate the affectivity of pharmaceuticals developed for its treatment.[7] Dox is a new class of chemotherapy that can increase the curative of lung cancer cells.[8] Polyphenols have assigned a special place in the nature in vivo/in vitro studies because of having multiple biological activities, such as: strong anti-oxidants, anti-inflammatory, anti-tumor. Quercetin with the chemical formula (C15H10O7) as one of the important members of the polyphenols group, has the following characteristics:[9] Free radical scavenger,[10] inhibitor of TNF-α,11 cyclooxygenase 2 and lipoxygenase
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