Evaluation of filling ability of Guttaflow Bioseal sealer to the simulated lateral canal by scanning electron microscope

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Background and objectives: Lateral canals establish a connection between the main root canal and periodontal ligament. Any necrotic tissue and bacteria residing in the lateral canals influence the efficiency of the filling of the root canal and the outcome of root canal therapy. The aim of this study was to determine which type of root canal sealer among GuttaFlow Bioseal, AH-Plus and Endosequence BC sealer provides deeper penetration into artificially prepared lateral canals. Methods: Thirty mandibular first premolars with single canals and mature roots were instrumented using Protaper universal rotary system after decoronation and standardisation of length (12mm). Three lateral canals were prepared in the coronal, middle and apical thirds in each root (at 3, 6, and 9 mm) using #15 engine-driven reamer. The specimens were divided into three groups (n = 10), according to the filling material, (I): Guttaflow Bioseal seal, (II): AH Plus sealer, (III): Endosequence Bioceramic sealer. In all groups, specimens were obturated using the corresponding Protaper universal gutta-percha and accessory cones. Specimens in each group were cross sectioned. Depth of sealer penetration was measured using a scanning electron microscope. Results: The data were subjected to ANOVA and Tukey’s HSD tests, with significance level of 5%. There was no difference among the tested sealers for filling the lateral canals. Concerning the positions of lateral canals, no differences were also found among sealer types, except for middle portion (at 7 mm from the apex); statistically there is a significant difference between three types of sealer (P value= 0.05), where GuttaFlow Bioseal showed a significantly greater penetration depth than AH Plus sealer.