Abstract
Sperm chromatin integrity is important for mammalian fertilization. The sperm chromatin structure assay (SCSA) using flow cytometry is frequently used for the assessment of fragmented sperm DNA. On the other hand, Toluidine blue (TB) assay is a simple alternate test for sperm chromatin assessment. The objective of our study was to evaluate the Toluidine blue test and SCSA for the assessment of sperm chromatin damage in proven and unproven donors.
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