Abstract
Resistance to b-lactam antibiotics by gramnegative bacteria, especially <em>Escherichia coli (E. coli)</em>, is a major public health issue worldwide. The predominant resistance mechanism in gram negative bacteria particularly <em>E. coli </em>is via the production of extended spectrum beta lactamase (ESBLs) enzymes. In recent years, the prevalence of b-lactamase producing organisms is increased and identification of these isolates by using disk diffusion method and no-one else is not satisfactory. So, this investigation focused on evaluating the prevalence of ESBL enzymes by disk diffusion method and confirmatory test (Combined Disk). Five hundred clinical samples were collected and 200 <em>E. coli </em>isolates were detected by standard biochemical tests. To performing initial screening of ESBLs was used from Disk diffusion method on <em>E. coli </em>isolates. A confirmation test (Combined Disk method) was performed on isolates of resistant to cephalosporin's indicators. Up to 70% isolates exhibited the Multi Drug Resistance phenotype. In Disk diffusion method, 128(64%) <em>E. coli </em>isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8%) isolates were detected as ESBLs producers. This survey indicate beta lactamase enzymes are playing a significant role in antibiotic resistance and correct detection of them in phenotypic test by using disk diffusion and combined Disk is essential for accurate recognition of ESBLs.
Highlights
Different strategies are used to protect bacteria from destructive effects of antibiotics.[1]
In Disk diffusion method, 128(64%) E. coli isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8%) isolates were detected as extended spectrum beta r lactamase (ESBLs) producers
This survey indicate beta lactamase enzymes are playing a the presence of clavulanic acid in compare absence clavulanic acid is increased, those organisms are recognized as a ESBLs producer.[10,11]
Summary
Different strategies are used to protect bacteria from destructive effects of antibiotics.[1]. In Disk diffusion method, 128(64%) E. coli isolates which resistant to ceftazidime and cefotaxime while in Combined Disk, among 128 screened isolates, 115 (89.8%) isolates were detected as ESBLs producers This survey indicate beta lactamase enzymes are playing a the presence of clavulanic acid in compare absence clavulanic acid is increased, those organisms are recognized as a ESBLs producer.[10,11] Complete detection of b-lactamase enzymes is essential for resistance control and successful treatment via the appropriate prescription of b-lactam drugs. The following antibiotics and concentration were used: cefotaxime (30 μg), ceftazidime (30 μg), gentamicin (10 significant role in antibiotic resistance and μg), amoxicillin (30 μg), imipenem (10 μg), correct detection of them in phenotypic test by using disk diffusion and combined Disk is Materials and Methods nalidixic acid (30 μg), streptomycin (10 μg), cotrimoxazole (1.25 μg), ciprofloxacin (5 μg) essential for accurate recognition of ESBLs. Bacterial strains and chloramphenicol (30 μg) (Mast Diagnostics Ltd., Bootle, Merseyside, UK). After 24 h incubation of sensitive category and reported as false negaperformed on isolates of resistant to plates at 37°C, ESBLs production was con- tive ESBLs which resulting from another facfirmed by ≥5 mm increase in the zone of inhi- tors such as AmpC that cover the effect of ESBL bition in the presence of clavulanic acid com- enzymes via creating resistance to clavupare to the absence of clavulanic acid.[13,14] lanate.[16,18]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
