↵​Evaluation of Estrona Powder as Estrus Inducer in Anestrus Buffaloes under Field Conditions

Abstract

Background: Anestrus is a multifactorial problem in buffaloes. Herbal preparations yielded favorable results with estrus induction. Hence, the present study was undertaken to evaluate the therapeutic efficacy of Estrona powder (Rakesh Pharmaceutical) on induction of estrus in anestrus buffaloes under field conditions. Methods: In total, 29 buffaloes were selected during Ambulatory clinics at village Sanoda and randomly allotted to two groups. The treatment group was fed 125g Estrona powder (n=18;101±10 Days in milk) for four days, while same amount of concentrate was fed to buffaloes of placebo group (n=11; 99±08 Days in milk). Blood from jugular vein was collected before initiation of treatment, day 0 (D0), day of estrus (DE) and on day 60 after AI/Service (D60) for estradiol (E2) and progesterone (P4) estimation. Result: A significant number of buffaloes in Estrona treatment group induced estrus 80%(13/18) within shorter time interval (11.3±4.9 days) as compared to Placebo group 27% (3/11). No effect of treatment was observed on E2 and P4 concentrations during D0, DE and D60. However, within group, there was significant effect on D0, DE and D60 on concentrations of P4 and E2 hormones. These results indicate that Estrona powder successfully induced estrus, hence can be used for estrus induction in buffaloes.