Evaluation of Endophytic Fungal Fractions of Phyllanthus amarus schum andthonn Leaves in Paracetamol and Ethanol Induced Hepatotoxicity

Abstract

Background Endophytes are the microbes residing inside the plant tissues forming relations such as mutualistic symbiotic and trophobiotic. Paracetamol is a widely used analgesic and antipyretic drug higher doses of which leads to serious liver disorders. Alcohol a psychoactive drug causes deleterious effects on the liver with high consumption. In both cases pathological conditions vary from basic intoxication to lifethreatening pathological states.nbspAim of the study An attempt was made to study the effects of fractions of endophytic fungi Nigrospora sp. CMH213 PALF-2 isolated in our previous report from leaves of Phyllanthus amarus in paracetamol and ethanol-induced hepatotoxicity.Materials and Methods In this study the effects of earlier isolated endophytic fungal fractions namely P2EA ethyl acetate and P2nB n butanol were studied on paracetamol and ethanol-induced hepatotoxicity at doses of 50 mgkg amp 100 mgkg. Various biochemical parameters and histopathology of the livers were also studied in both models.Results In both the models the elevated biochemical parameters such as aspartate aminotransferase AST alanine aminotransferase ALT alkaline phosphatase ALP total bilirubin direct bilirubin and triglycerides TG were reversed by P2EA and P2nB 50 mgkg amp 100 mgkg as compared to induced groups plt0.001. P2EA and P2nB 50 mgkg amp 100 mgkg also restored the lipid peroxidation LPO superoxide SOD and catalase CAT levels.Conclusion P2EA and P2nB exhibited hepatoprotective activity significantly in paracetamol and ethanolinduced models from endophyte Nigrospora sp. CMH213 PALF-2 from Phyllanthus amarus...nbsp