Evaluation of endocrine disruptors in male ruminants: Study of toxic effects of Ipomoea carnea in goats

Abstract

s / Toxicology Letters 229S (2014) S40–S252 S43 Purpose: A range of different in vitro chemistry-based (DPRA, GSH reactivity) and cell-based methods (MUSST, hCLAT, Keratinosens) have been developed and some of them are currently evaluated for their applicability to cosmetic ingredients and their physicochemical diversity. Although these assays appear to be promising for hazard identification, potency assessment is still limited. Possible limitationsmay be linked to themetabolism thatmay differ between the models and native skin, to the bioavailability which is not considered in monolayer cultures, and to the danger signal thatmay be different inmonolayers as compared to a natural tridimensional microenvironment. Method: We have developed SENS-IS, a method, based on the quantitative analysis of specific biomarkers expressed in 3D reconstructed epidermis (Episkin), thus providing a possible way to encompass these limitations and come closer to potency assessment. The aim of this study was to confirm the transferability and reproducibility of the SENS-IS protocol and its ability to correctly classified sensitizing potency in 5 classes similarly to the LLNA. Results and conclusions: Three laboratories participated in this SENS-IS ring study using 19 chemicals tested blindly. All chemicals were similarly classified by the 3 laboratories with exception using HCA that was overestimated by one laboratory. Analysis of predictive capacity (with 5 class) was 84% as compare to LLNA and 100% with one class difference. These results shows that the SENS-IS assay can be steadily transferred and provides reproducible results on safety assessment of chemical products. http://dx.doi.org/10.1016/j.toxlet.2014.06.189