Evaluation of Electrical Conductivity of Hemoglobin and Oxidative Stress in High Fat Diet Rabbits

Abstract

Abstract: The aim of this study was to evaluate the effects of feeding hgh cholesterol and saturated fat diet for a period of 10 weeks on the antioxidant status and the electrical conductivity of hemoglobin in rabbits. Thus, twenty of 12 weeks old male New Zealand whte rabbits obtained from the Laborato~y Animal Centre (College of Pharmacy, King Saud University, Saudi Arabia) were used. The rabbits were indvidually caged and divided into control group and high fat diet group. Sem lipids were measured using standard techniques. The electrical conductivity of hemoglobin and oxidative stress were evaluated in both groups of rabbits. We found that the levels of Total Cholesterol (TC), Triglycerides (TG), Low Demity Lipoprotein (LDL), High Density Lipoprotein (HDL) and Malondaldehyde (MDA) were significantly increased in the hgh fat diet rabbits compared with the control rabbits, and a significant decrease in the activities of plasma antioxidant enzymes, such as Superoxide Dismutase (SOD) and Glutahone Proxidase (GPx), was obsenred in the high fat det rabbits compared with the control rabbits. The electrical conductivity of hemoglobin was significantly increased in the high fat det rabbits compared with the control rabbits. Furthermore, a significant increase in the oxidative stress was obsenred in the high fat diet rabbits compared with the control rabbits, which was concomitant with the increase in the electrical conductivity of hemoglobin. Our results suggest that feedng rabbits a high cholesterol and saturated fat diet for a period of 10 weeks induces significant changes in TC, TG, LDL, HDL, MDA, SOD, GPx, the electrical conductivity of hemoglobin and the oxidative stress. Indeed, SOD, GPx, the electrical conductivity of hemoglobin and the oxidative stress may help in diagnosing and monitoring the progression of atherosclerosis.