Abstract
The International Scientific Research Organization for Science, Engineering and Technology (ISROSET) is a Non-Profit Organization; The ISROSET is dedicated to improvement in academic sectors of Science (Chemistry, Bio-chemistry, Zoology, Botany, Biotechnology, Pharmaceutical Science, Bioscience, Bioinformatics, Biometrics, Biostatistics, Microbiology, Environmental Management, Medical Science, Forensic Science, Home Science, Library Science, Material Science Military Science, Physical Science, Physical Education Science, Educational Science, Fisheries, seed technology, Agriculture, Forestry Science, Mathematics, Physics, Statistics and Geology/Earth Science), Computer Science, Engineering and Information Technology, Commerce, Management, Economics Sociology and Social Science.
Highlights
Since the cost of large-scale sequencing and genotyping is considerably less in recent years, there is increasing trends in applying these methods to various human populations, to investigate questions concerning forensics, population history, migrations, local adaptation, etc., [1]
The Scheffe multiple comparisons performed at the 0.05 significance level found that the mean yield of genomic DNA extracted from saliva (Oragene, OG500) (M = 362.70, SD = 28.23, N = 10) was significantly greater than all other sample types in this study
There was statistically significant difference in the mean yield of DNA from buccal cells collected by Puritan foam swab (M = 43.86, SD = 8.15, N = 10) than HiMedia (M = 17.36, SD = 3.06, N = 10) foam swab and dried blood collected on NucleoSave (M = 0.7, SD = 0.24, N = 10)
Summary
Since the cost of large-scale sequencing and genotyping is considerably less in recent years, there is increasing trends in applying these methods to various human populations, to investigate questions concerning forensics, population history, migrations, local adaptation, etc., [1]. A critical requirement for such large-scale population studies or for the forensic purpose is the quantity and quality of DNA extracted from the sample. DNA testing of the evidence collected from a crime scene, biological samples should be obtained from suspects. It is very important to maintain the integrity of the reference forensic samples until DNA analysis is performed. Obtaining blood sample is an invasive procedure that requires training in phlebotomy, it is painful for the donor and there are chances of infection too. These constraints limit the suitability of blood collection as a reference sample for some populations, which in turn has led to a search for alternative sources of DNA. Many laboratories often use cheek swabs/ buccal swabs collection rather than drawing blood
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