Abstract

The glutathione reductase (GR) of baker's yeast (Saccharomyces cerevisiae) was exposed to titanium dioxide nanoparticles (TiO2 NPs) and zinc oxide nanoparticles (ZnO NPs) at concentrations of 0, 25, 50, 100, 250, and 500mg/L (ppm) in vitro. According to the calculations, the effect of 25, 50, 100, 250, and 500mg/L TiO2 NPs on GR enzyme activities resulted in percentage changes of - 3.12; - 0.87; - 2.12; - 2.12, and - 1.50, respectively. Percentage changes in GR enzyme activities with the effect of 25, 50, 100, 250, and 500mg/L ZnO NPs were calculated as - 2.01; + 1.88; + 0.38; - 2.51, and + 0.75, respectively. It can be deduced from this research that the change in concentrations of TiO2 NPs and ZnO NPs has no statistically significant effect on GR enzyme activities in comparison with the control groups (p > 0.05, N = 3).

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