Evaluation of effectiveness and minimum inhibitory concentration of silver nanoparticles for decontamination of root canal systems

Abstract

Endodontics is essential in the process of controlling pain and diseases of the pulp and periapex, thus becoming increasingly innovative, safe and effective. Endodontic treatment has several stages, including the process of canal evolution. This decontamination process is extremely important for the eradication of microorganisms in the SCR and the prevention of reinfection. During the instrumentation it is paramount that constant irrigation occurs to remove inflamed tissues, necrotics and also the biofilm present. The objective of the present study was to analyse the disinfectant capacity of two chemical substances, one of them in different concentrations, o n an aggressive microorganism present in the root canal system. The applied methodology was, in vitro experimental laboratory study, to compare the antimicrobial activity of 1% Sodium Hypochlorite and Silver Nanoparticle at 22 ppm, 70 ppmand 95 ppm, used against the pathogen E. faecalis, which were selected by the bank of microorganisms from the laboratory of Molecular and Clinical Microbiology of the Graduate Program in Health Sciences at Universidade São Francisco (USF), as well as determining the minimum concentration of the silver nanoparticle solution in inhibiting the growth of E. faecalis and P. aeruginosa. Which storage was previously authorized by the Research Ethics Committee of the USF. After data collection, it was possible to conclude from the results that the 1% Sodium Hypochlorite, the Silver Nanoparticle at 70 ppm and 95 ppm, obtained positive results in terms of antimicrobial activity, in comparison with the Silver Nanoparticle at 22 ppm, which obtained a negative result on the microorganism.