EVALUATION OF EFFECT OF THE STORAGE TIME ON PLATELET CONCENTRATION IN PLATELET RICH FIBRIN- BIOCHEMICAL AND MICROSCOPIC ANALYSIS

Abstract

Aim of the present study was to evaluate the effect of storage time on platelet-rich fibrin (PRF) and the stability of the fibrin clot over a period of time. Three blood samples were drawn from participants in sterile blood sampling tube. Two blood samples drawn in sterile glass tubes were centrifuged to form PRF. Third non-centrifuged sample drawn in EDTA-containing tube was used to measure baseline blood platelet concentration. After PRF had formed, it was removed from respective test tubes at different time intervals i.e. immediately after centrifugation- PRF A (Group A) and after 60 min of storage time in the blood collecting tube- PRF B (Group B). Residual blood from each group was tested for platelet concentration and compared with baseline reading. Similarly, PRF membranes were studied microscopically immediately after centrifugation and other after 60 minutes. Platelet concentration of PRF in blood and PRF membrane for both groups was calculated using the difference between baseline and residual platelet concentration- biochemically and microscopically. A paired t-test showed a statistically significant difference between the two groups (p<0.005).