Abstract
The alkaloids such as echitamine, ditamine and echitenine were subjected for anti cataract activity using in vitro cataract model. Goat lenses were incubated in artificial aqueous humor containing 55 mM glucose with test alkaloids in different concentrations at room temperature for 72 h. Studied Biochemical parameters were sodium ion, potassium ion level and Na+-K+-ATPase activity. Glucose induced opacification of goat lens began 8-10 hrs after incubation and was complete in 72-80 hrs. Cataractous lenses treated with echitamine shown lower sodium ion, higher the potassium ion and higher Na+-K+-ATPase activity. Echitamine treated lens prevented formation and progress of cataract as evidenced by biochemical parameters and visual observation. Ditamine and echitenine were devoid of anti - cataract activity.
Highlights
Cataract, the clouding of the lens develops later in the life and it is most likely the consequence of decades of accumulated damage to long lived lens protein
There are no any prophylactic approaches are available to prevent cataract formation, none evaluated found to be effective. We evaluated alkaloids such as echitamine, ditamine and echitenine for anti cataract activity using in vitro cataract model
Visual evaluation shown that the Group II containing glucose 55 mM and Echitenine and Ditamine treated group in all concentration of Group III, has shown opacification starting after 8 hrs at the periphery, on the posterior surface of the lens
Summary
The clouding of the lens develops later in the life and it is most likely the consequence of decades of accumulated damage to long lived lens protein. It is the most common cause of blindness. Oxidative mechanism plays an important role in biological phenomena, including cataract formation. The formation of superoxide radicals in the aqueous humor and in lens, lens and its derivatization to other potent oxidants may be responsible for initiating various toxic biochemical reactions leading to opacification (Harding and Rixon., 1980). The aldose reductase enzyme plays an important role in pathogenesis of cataract. The aldose reductase acts on glucose, galactose and xylose and Lokesh J Shetty / Journal of Applied Pharmaceutical Science 5 (05); 2015: 065-067
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