Abstract
The dried blood spot (DBS) sampling has a lot of advantages in comparison with the “standard” venous blood collecting, such as small collection volume, painless and easy sample collection with minimal training required, stable and transportable at ambient temperatures, etc. The aim of this study was to determine the comparability of four different types of DBS sampling (HemaSpot™-HF Blood Collection Device, Whatman® 903 Protein Saver Snap Apart Card, card ImmunoHealth™, and glass fiber strip ImmunoHealth™) for analysis of the global metabolites profile. All the samples were collected from the same person at the same time and stored at room temperature for four weeks in order to exclude all possible deviations deriving from biological variances and to evaluate sample storage stability. Metabolome profiling by direct injection of a deproteinized capillary blood DBS sample into an electrospray ion source of a hybrid quadrupole time-of-flight mass spectrometer was used. Differences in the metabolomics profile were found between the different DBS collection materials, especially for ImmunoHealth™ card and ImmunoHealth™ glass fiber strip. However, our results indicate that the analytical performance of all tested DBS sampling materials showed consistent results overall detected metabolites and no dramatic changes between them in the metabolic composition during the storage time.
Highlights
Blood is one of the most used biological fluids for research, diagnostic, health, and drug monitoring.Following the trend of development of precision medicine with emphasis on personalized approach and population health studies, tools that facilitate sample collection and enable patients to contribute to the expanding bioanalytical empowerment for health care are necessary
To evaluate the best dried blood spot (DBS) sampling for metabolomics application, we analyzed the metabolomes extracted from HemaSpotTM-HF Blood Collection Device, Whatman® 903 Protein Saver Snap Apart
Card ImmunoHealthTM, and glass fiber strip ImmunoHealthTM by using a direct injection mass spectrometry (DIMS) method as a typical workflow designed for rapid untargeted analysis of the polar blood metabolome
Summary
Blood is one of the most used biological fluids for research, diagnostic, health, and drug monitoring.Following the trend of development of precision medicine with emphasis on personalized approach and population health studies, tools that facilitate sample collection and enable patients to contribute to the expanding bioanalytical empowerment for health care are necessary. A small collection volume is required for DBS: less than 100 μL of capillary blood is needed to spot onto sampling paper compared to a minimum of 0.5 mL of blood in venous sampling. DBS samples are stable at ambient temperatures and offer possibilities for easy shipment and storage without the expense and infrastructure associated with maintenance of specific temperature (e.g., no requirement for freezing or dry ice using). Using this methodology, blood samples can be collected by the patient itself or his guardian with minimum training and sent by mail to the assigned laboratory. Clinical trials or cohort studies requiring collection, shipment, storage, and analysis
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