Abstract
A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH2–3 generations. Well established DH lines from MRP5401 in DH3 generation had early flowering and yield gain.
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