Abstract
In the underlying study we evaluated different single-use filtration systems for cell separation and harvest clarification in 1,000 L scale. A screening of different depth filters was carried out with various single-use filters from Pall, Cuno (3M), Millipore and Sartorius Stedim. In total, we included 85 depth filtrations in the screening. Out of that, two single-use filtration systems were chosen and further tested in 200 L scale. Based on these results, a single-use filtration set-up for harvesting production scale fed batch processes was determined.
Highlights
In the underlying study we evaluated different single-use filtration systems for cell separation and harvest clarification in 1,000 L scale
Material and methods High cell density fed batch cultivations of a monoclonal antibody expressing Chinese Hamster Ovary (CHO) cell line were harvested by depth filtration and Retention range* 20 – 4
Filtrate turbidity was below 7 NTU when applying PDK6, whereas in filtrates generated with the coarser filter PDK7 turbidities up to 9 NTU were observed
Summary
In the underlying study we evaluated different single-use filtration systems for cell separation and harvest clarification in 1,000 L scale. A screening of different depth filters was carried out with various single-use filters from Pall, Cuno (3M), Millipore and Sartorius Stedim. We included 85 depth filtrations in the screening. Two single-use filtration systems were chosen and further tested in 200 L scale. Based on these results, a single-use filtration set-up for harvesting production scale fed batch processes was determined
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