Abstract
A new ultraviolet (UV) light source EBE-UV (electron beam excitation-UV) system was established in this study, which can emit UV light at four wavelengths (233/243/260/270 nm) simultaneously. The EBE-UV lamp emits deep ultraviolet light by electronically exciting the ultraviolet material YPO4:Pr3+. It has the advantage of being mercury-free and environmentally friendly, as well as higher photoelectric conversion efficiency. To evaluate the germicidal performance of the EBE-UV lamp, Gram-negative bacteria, Gram-positive bacteria, and fungi were selected for evaluation. The bactericidal efficiency of the EBE-UV lamp was tested and compared to the universal 255 nm and 275 nm UV-LEDs. As a result, the bactericidal effect of EBE-UV light was more effective than of single-wavelength UV-LEDs, especially against fungi. The level of Reactive Oxygen Species in the cells of the strain was increased to varying degrees by UV irradiation. There is a general weakening of energy metabolism, and DNA was damaged and fragmented. However, almost no significant cell membrane damage was found in this study. The EBE-UV light will show great potential due to its disinfection effect and device performance.
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