Evaluation of different extraction methods on antimicrobial potency of Adenium obesum stem against food borne pathogenic bacterial strains in Oman

Abstract

ObjectiveTo determine and compare the effect on antimicrobial potency of crude stems extract of Adenium obesum (A. obesum) by Soxhlet and maceration extraction methods. MethodsThe crude extracts were prepared from the coarse samples of stems with methanol by using Soxhlet and maceration extraction methods. Both the crude extracts from two extraction methods were dissolved in water and successively extracted by different polarities solvents with increasing polarities. In vitro antimicrobial potency of different polarities crude extracts obtained from Soxhlet and maceration methods was determined by agar gel diffusion method against different food borne pathogenic bacterial strains. ResultsThe results for antimicrobial potency of different crude extracts were almost similar by Soxhlet and maceration and methods. The average range of inhibition potency of different polarities crude extracts was 0%-17% by Soxhlet method and inhibition potency 0%-24% by maceration method. ConclusionsThese results obtained from in vitro approach give promising basic information about this plant as well as some potential crude extracts can be used for the treatment of infectious diseases.