Abstract
Bacteria of the genus Alicyiclobacillus spp. form spores and develop in acid media, leading to the spoilage of citrus juices. Brazil is the largest exporter of orange juice concentrate, and yet, it has been extensively studied due to changes in taste and smell. Several investigations have reported different culture media used to detect and enumerate Alicyiclobacillus spp. The objective of the present study was to evaluate the recovery of Alicyiclobacillus spp. spores grown in ALI, BAT, K agar and YSG media using the methodology suggested by ABECitrus. Five inocula were used, two from reference strains and three from pasteurized concentrated orange juice. Cell recovery after the enrichment in reconstituted orange juice was also analyzed. An initial population of 6 log CFU/mL was inoculated. ALI, BAT and YSG media were able to recover the initial population of all different inocula, with no significant differences between the results. When compared to BAT, however, the preparation of ALI and YSG media was simpler and had more advantages. The recovery with K agar medium was lower than the other media for all the tested inocula, with significant differences found for Alicyclobacillus acidocaldarius 0298T (3.66 log CFU/mL) and Alicyclobacillus pomorum-like CBMAI 0278 (4.11 log CFU/mL).
Highlights
The genus Alicyclobacillus ssp. is comprised of bacteria which grow in acid media at high temperatures
An initial population of 6 log CFU/mL of each micro-organism was inoculated on the different media to verify recovery
The germination results found for the medium of K agar were lower than the other media, no significant differences were found in the assays performed with CBMAI 0244T, CBMAI 0281 and CBMAI 0294
Summary
The genus Alicyclobacillus ssp. is comprised of bacteria which grow in acid media at high temperatures. Is comprised of bacteria which grow in acid media at high temperatures. They multiply in different types of food with pH lower than 3.7, such as tomatoes, citrus fruits, and their products (Hippechen et al, 1981). Several studies have been performed on new alternatives to prevent orange juice spoilage caused by bacteria such as the genus Alicyclobacillus spp. Alicyclobacillus spp. are gram-positive, rod-like bacteria, whose cell membrane is mainly composed of cyclic fatty acids. They are spore-forming bacteria resistant to pasteurization processes employed in the production and reconstitution of concentrated orange juice. Some species of Alicyclobacillus, like A. acidoterrestris and A. acidocaldarius, may exhale an anti-septic odor due to the formation of guaiacol (Gocmen et al, 2005)
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