EVALUATION OF DIAGNOSTIC SIGNIFICANCE OF MOLECULAR-GENETIC MARKERS IN РАNCREATIC ADENOCARCINOMA

Abstract

The purpose of the study : to evaluate the diagnostic value of molecular genetic markers in pancreatic cancer. Subjects. Data on patients with pancreatic cancer and patients with chronic pseudo tumor-like pancreatitis. The point mutations in K-ras gene were analyzed using polymerase chain reaction (PCR); the mutations in BRCA1, 2 genes were determined, the status of methylation of promoter segment of р16INK4A gene was analyzed. 67 % patients with pancreatic cancer and 63,6 % patients with chronic pancreatitis detected the mutant K-ras gene. 6,7 % patients with pancreatic cancer revealed the BRCA1 gene mutations in the heterozygous state, 13 % patients revealed the gene BRCA2 mutations. Diagnosis of the K-ras gene mutations in patients with pseudo tumor-like pancreatitis is conjugated with high risk of transformation of the pancreas to adenocarcinoma. 41 % patients with pancreatic cancer detected aberrant hypermethylation of р16INK4A gene, which is indicative of its aggressive genotype and poor prognosis.