Abstract
This study aims to compare the performance of lineprobeassay (LPA) onsmear-negative samples with that of smear-positive samples for diagnosingpulmonarytuberculosis (PTB) andfirst-line drugsensitivitytesting (FLDST). A total of 196 sputum samples including both smear-positive (112) and negative (84) samples of patients suspected of PTBwere subjected to LPA for TB detection and FL DST. TB culture followed by MPT 64 Ag was done and conventional FL DST was performed on all culture-positive isolates. Results of LPA on smear-negative were compared with smear-positive samples. The LPAconfirmed the diagnosis of PTB in 104/112 smear-positive cases but in only 36/84 smear-negative cases. The assay had 47.36%, 72.72%, and 88.88% sensitivity and 86.96%, 95.23%, and 95.65% specificity in smear-negative cases compared to 89.09%, 95.83%, and 98.07% sensitivity and 100%, 98.36%, and 98.24% specificity in smear-positive cases for detecting Mycobacterium tuberculosis (MTB), rifampicin (RMP)resistance, and isoniazid(INH) resistance, respectively. LPA performance was better on smear-positive than smear-negative sputum samples. Further larger studies are needed to justify the use of LPA on smear-negative pulmonary samples for diagnosis.
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