Abstract
The aim of the present study was to screen the genotype profile of bovine DGAT1 gene among Gir and Kankrej cattle in Gujarat, India. Total number of 200 Gir cattle and 100 Kankrej cattle were evaluated for CfrI-RFLP based genotyping of DGAT1 gene. We observed that only two genotypes (KK and KA) exist among the studied population with the alleles frequency of 0.925 (n=170) and 0.075 (n=30), respectively for K and A in Gir cattle as well as 0.895 (n=79) and 0.105 (n=21) respectively in Kankrej cattle. The overall calculated allele frequency for K and A was 0.915 and 0.085, respectively in both zebu (Bos indicus) cattle. Association of genotypes with milk production traits revealed that KA had significant (P<0.05) effect on total milk yield, as compared to KK in Gir and Kankrej cattle breed. Similarly, KK genotype had significant (P<0.05) effect on fat percentage, as compared to KA in Gir and Kankrej.
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