Abstract
Cottonseed is potential candidate to act as an important protein source but presence of toxic compound gossypol with concentration more than 0.045% is main hurdle in utilization of this protein. Optimized lyophilized protein powder constitutes 5.05% moisture, 93.1% crude protein, 0.03% free gossypol, 0.27% total gossypol, and total bacterial count of 1295 cfu/g with no presence of pathogenic bacteria. Cottonseed may be utilized as an ingredient for food industry as a supplement in various foods since it has surpassed all critical quality standards except lysine content as mentioned in Food Safety and Standards Regulations, 2011 (FSSR, version IV, 2017).
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