Abstract
Antimicrobial peptides (AMPs) can be found in various organisms, and could be considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens.
Highlights
Plant diseases have a great impact on the productivity of several crops, and their control is often accomplished only by excessive use of agrochemicals
Through the criteria used for prospecting putative Antimicrobial peptides (AMPs) in citrus sequences, peptides obtained from hypothetical protein of Citrus sinensis and Pyruvate Kinase protein from Citrus aurantium were chosen
The antimicrobial and hemolytic activities were determined to evaluate the effect of these AMPs against bacterial growth and eukaryotic cells
Summary
Plant diseases have a great impact on the productivity of several crops, and their control is often accomplished only by excessive use of agrochemicals. Antimicrobial peptides (AMPs) have been used as a new strategy for the control of plant diseases, due to the need for alternatives with less or no toxicity effect and impact on the environment [2]. Antimicrobial peptides are a group of molecules conserved during evolution that can be found in a wide range of organisms [3,4]. Most of these peptides have similar characteristics: (a) they are cationic with two or more positive charges, (b) contain from ten to fifty amino acid residues, (c) have 50% hydrophobic residues, and (d) form α-amphipathic helix [5,6]
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