Evaluation of cytogenotoxic potential and embryotoxicity of KRS-Cauvery River water in zebrafish (Danio rerio)

Abstract

In the Cauvery River (CR), indiscriminate discharge of waste causes unexplained skeletal deformity in some fish species present in the water. To investigate this phenomenon, we analyzed the biological, physical, and chemical parameters present in the water and then evaluated the toxicity effects on the zebrafish (Danio rerio) model. The zebrafish were treated with KRS-CR water samples collected from three stations (fast-flowing water [X], slow-flowing [Y], and stagnant [Z] water), before and after filtration. Firstly, we detected microscopic organisms (MO) such as Cyclops, Daphnia, Spirogyra, Spirochaeta, and total coliform (Escherichia coli), which are bioindicators of water pollution present in the samples. All physicochemical parameters analyzed, including heavy metals before and after filtration of the water with Millipore filter paper (0.45 µm), were within the acceptable limits set by standard organizations, except for decreased dissolved oxygen (DO), and increased biochemical oxygen demand (BOD), and chemical oxygen demand (COD), which are indicators of hypoxic water conditions, as well as the presence of microplastics (polybutene (< 15 µm), polyisobutene (≤ 20 µm), and polymethylpentene (≤3 mm)) and cyclohexyl in CR water samples. Zebrafish embryos treated with the water samples, both before and after filtration exerts the same cytogenotoxic effects by inducing increased reactive oxygen species (ROS) production, which triggers subcellular organelle dysfunctions, DNA damage, apoptosis, pericardial edema, skeletal deformities, and increased mortality. As a result, we observed that both water samples and zebrafish larvae had significantly less oxygen using SEM and EDS. Our findings show that KRS-CR water can induce cytogenotoxic and embryotoxic defects in zebrafish due to hypoxic water conditions triggered by the microplastics influx. The present study would provide valuable insights for health hazards evaluation and future river water treatment strategies.