Abstract
Publisher Summary This chapter analyzes the effectiveness of cytochrome P450-4 ω -hydroxylase and 20-hydroxyeicosatetraenoic acid (HETE) as an O 2 sensing mechanism in the microcirculation. In contrast to the vasodilation occurring in response to reduced PO 2 , increasing tissue oxygen delivery elicits a vasoconstrictor response in most peripheral vascular beds. This enables the microcirculation to normalize blood flow during increases in tissue perfusion and to shunt flow from areas of tissue that are oxygenated adequately to regions that require additional blood flow. In this study, the role of 20-HETE in regulating vascular tone is discussed. Expression of P450 protein and isoforms in the vasculature, thepotential role of 20-HETE in mediating vascular responses to increased oxygen availability, the experimental method for determination of the contribution of 20-HETE to the constriction of arterioles to elevated PO 2 in the microcirculation in situ are discussed.A method for determination of the contribution of 20-HETE to responses of isolated resistance arteries to changes in PO 2 and concepts related to the identification of cytochrome P450-4A isoforms in isolated cremasteric arterioles and parenchymal tissue by reverse transcriptase–polymerase chain reaction (RT-PCR) are also described.
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