Evaluation of critical points of mould growth and mycotoxin production in the stored barley ecosystem with a hazardous initial microbiological state of grain

Abstract

The development of effective control systems for post-harvest preservation and storage processes seems to be one of the best ways to reduce the activity of fungi and thus the risk of mycotoxin contamination of grain during its storage. A crucial step towards developing such a system requires the understanding of ecological relationships between environmental factors, fungal development and mycotoxin production in bulk of grain treated as holistic systems. The aim of this study was to estimate the critical points associated with mould development and mycotoxin production in the ecosystem of malting barley grain with a hazardous initial microbiological state (provided by the inoculation of naturally contaminated grain with Aspergillus ochraceus and Penicillium verrucosum) stored in different temperature and water activity conditions (t = 12–30 °C and aw = 0.78–0.96, where aw corresponds to equilibrium relative air humidity in inter-grain spaces). The most intensive fungal growth occurred in the ecosystems of barley grain with aw = 0.91–0.93 stored at 24 and 30 °C. The biosynthesis of ochratoxin A (OTA) was observed at 0.91–0.95 aw at 18–30 °C, whilst penicillic acid and citrinin were detected only at temperatures of 24 and 30 °C. The toxicogenic ability of fungi at 12–24 °C and aw≤0.80 was significantly limited. In the ecosystem of barley grain, the threshold of fungal concentration (expressed in colony forming units of moulds, CFU g−1), for which the amount of OTA exceeded binding limits, was 105 CFU g−1 of grain. The study indicates that the duration of the lag phase of fungal development (comprising spores germination and the beginning of exponential mould growth), which in all tested ecosystems was shorter than the time, after which admissible levels of OTA were exceeded, may be used to evaluate the safe period, at which grain should be subjected to preservation processes.