Abstract
Objective: To Evaluate cortisol level before and after insertion of orthodontic appliances at different time interval among the subjects, females, males and in between both the groups on comparison. Materials and Methods: Total number of 20 north Indian subjects of the age group between 15 and 30 years were randomly selected for the study after meeting the study criteria and samples were collected before bonding (T0), 1 hour after bonding (T1) and 4-6 weeks after bonding (T2). The method of analysis used for the study is chemiluminescence assay (CLIA). Evaluation of cortisol level was done using immunoassay kits. Result: There was a significant decrease in serum cortisol from pre-operative to 1 hour and 4 weeks and same for the males group and female group. There is no significant difference in the level of saliva cortisol on comparison in between male and female group. Conclusions: Fixed orthodontic appliances did show a significant effect on salivary parameters in our study that was saliva cortisol level which was related to inflammation or stress.
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