Abstract
Using quantitative receptor autoradiography (QAR), it is possible to investigate the distribution of ligand binding sites as well as binding affinity. Accuracy in this technique is critically dependent on the quality of available radioactive calibration standards. For autoradiography using tritiated ligands, commercial 3H standards are available. These are also used, after cross-calibrating, as convenient standards for short-lived radioisotopes for which commercial standards are not available. A common practice in QAR is to appose these standards, each a slide containing 14 plastics of known radioactive concentration, along with the tissue sections on each sheet of x-ray film and to use these to produce a calibration curve and assign absolute concentration to densitometric measurements taken from the film. Typically, experimenters use multiple standards in their studies and directly compare data calibrated using different calibration sets. To our knowledge, only one study has tested the implicit assumption that the standards, once corrected for decay, are equivalent. That study concluded that the variability among standards is low. We revisit the issue.
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