Abstract

AbstractSince poor quality inoculants could be one of the factors responsible for nodulation failures in soybeans [Glycine max (L.) Merr.], 93 planter‐box inocultants from commercial retail sources in South Carolina and Georgia were evaluated for numbers of viable Rhizobium japonicum on yeast extract mannitol agar. Spread plates were counted after incubation for 7 days at 28 C Two sub‐samples from each commercial inocultant were evaluated. Viable rhizobial counts ranged from 6.9 × 109 to ≤ 1.0 × 103/g of inoculant. Statistical differences were detected among brands. Inoculants which contained a fungicide and/or Mo were consistently and significantly lower in viable rhizobia than other brands. The low viable rhizobial counts were also reflected in poor nodulation of soybean roots under greenhouse conditions. A highly significant correlation was obtained between the log of viable rhizobia and the number of nodules per plant for 27 inoculants. Plate counts provided a relatively rapid and inexpensive method to assess the quality of commercial inoculants.

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