Evaluation of Chitosan Derivatives Modified Mesoporous Silica Nanoparticles as Delivery Carrier

Qi Li,Xianlan Cui,Gaowei Hu,Dejun Sun,Zheng Jin,Kai Zhao,Wenqian Wang

doi:10.3390/molecules26092490

Qi Li, Xianlan Cui + Show 5 more

Open Access

https://doi.org/10.3390/molecules26092490

Copy DOI

Abstract

Chitosan is a non-toxic biological material, but chitosan is insoluble in water, which hinders the development and utilization of chitosan. Chitosan derivatives N-2-Hydroxypropyl trimethyl ammonium chloride (N-2-HACC) and carboxymethyl chitosan (CMCS) with good water solubility were synthesized by our laboratory. In this study, we synthesized mesoporous SiO2 nanoparticles by the emulsion, and then the mesoporous SiO2 nanoparticles were modified with γ-aminopropyltriethoxysilane to synthesize aminated mesoporous SiO2 nanoparticles; CMCS and N-2-HACC was used to cross-link the aminated mesoporous SiO2 nanoparticles to construct SiO2@CMCS-N-2-HACC nanoparticles. Because the aminated mesoporous SiO2 nanoparticles with positively charged can react with the mucous membranes, the virus enters the body mainly through mucous membranes, so Newcastle disease virus (NDV) was selected as the model drug to evaluate the performance of the SiO2@CMCS-N-2-HACC nanoparticles. We prepared the SiO2@CMCS-N-2-HACC nanoparticles loaded with inactivated NDV (NDV/SiO2@CMCS-N-2-HACC). The SiO2@CMCS-N-2-HACC nanoparticles as delivery carrier had high loading capacity, low cytotoxicity, good acid resistance and bile resistance and enteric solubility, and the structure of NDV protein encapsulated in the nano vaccine was not destroyed. In addition, the SiO2@CMCS-N-2-HACC nanoparticles could sustain slowly released NDV. Therefore, the SiO2@CMCS-N-2-HACC nanoparticles have the potential to be served as delivery vehicle for vaccine and/or drug.

Highlights

Drug delivery agents can regulate the distribution of drug in the body in terms of delivery space, time and dosage
Li et al used mesoporous silica nanoparticles as a delivery carrier, co-loaded anti-angiogenesis agents and chemotherapeutic drugs, and combined with targeting molecules for anti-angiogenesis and chemotherapy, the results showed that the dual-load drug delivery system could deliver the drugs in tumor blood vessels and entered into the tumor through a differentiated drug release strategy, thereby enhancing the anti-tumor effect [34]
The mesoporous SiO2 nanoparticles synthesized under the optimal process conditions were spherical in shape and uniform in size (Figure 2A,B)

Summary

Introduction

Drug delivery agents can regulate the distribution of drug in the body in terms of delivery space, time and dosage. The goal is to deliver the right amount of medicine to the right place at the right time to increase the bioavailability of the medicine and reduce costs and side effects [1,2,3]. The emergence of mesoporous structural materials has attracted the attention and research of scientists [4]. Mesoporous SiO2 nanoparticles have been proved to be the most promising and potential materials in the field of biomedicine by virtue of their unique porous structure and excellent biocompatibility [5,6]. Mesoporous SiO2 has a typical honeycomb channel structure and significant loading and retention capabilities.

Methods

Results

Conclusion