Evaluation of Chitinase producing and antimicrobial properties of streptomyces isolated from shrimp shell disposable area

Abstract

ObjectiveAt present scenario need the potential medical application enzymes and control the major clinical bacterial pathogens produce infections in humans. In this mind isolate and characterization of chitinase and antibacterial compounds from streptomyces. MethodsThe actinobacterial strains isolated from crab and shrimp shells disposable area (Parangipettai) were screened for chitinolytic activity on colloidal chitin-agar plates. After incubation the clear zone producing strain were selected for chitinase production. Every 2 days interval chitinolytic activity was measured and protein estimation was determined. Antagonistic activity of chitinase producing actinobacterial isolates were tested by adopting agar plug method. Based on the preliminary screening results, chitinase producing strain was used for bioactive substance production through submerged fermentation by adopting shake flask method. Antibacterial activity of crude extracts was tested by disc diffusion method at 100μg/disc concentration. Resultstotally 15 actinobacterial strain were isolated. Based on the zone formation on chitin minimal plate CDB20 showed promising zone activity. The CDB20 strain was inoculated in chitin production medium at 10 days. After incubation the chitinase activity showed 1.22U/ml protein estimation 14mg/ml. The preliminary screening showed promising antibacterial activity against clinical bacterial pathogens. Secondary screening results showed maximum K. pneumonia(23mm) and minimum S. aureus (18mm) was observed. The potent strain identified as genera streptomyces. ConclusionThe isolated potent streptomyces have degraded chitin and inhibited the clinical bacterial pathogens. In future in this strain will be used for waste shrimp and crab shells management and recycling and In this strain produce chitinase and antibacterial compounds have more medical application. So this strain has multi functional applications.