Abstract
<p>The present study was conducted to evaluate the antioxidant activity and chemical composition of aqueous methanolic extract of <em>Trichodesma indicum </em>(L.) R.Br. The extract was tested for antioxidant activity using various <em>in vitro</em> models viz., DPPH, ABTS, NO, FRAP, total antioxidant activity and reducing power. The total phenolic and flavonoid contents were found to be equivalents to 97.83 ± 0.08 µg of gallic acid and 45.45 ± 0.19 µg of rutin /mg of dried aqueous methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed in the ABTS (IC<sub>50</sub> 17.13 ± 0.23 μg) followed by DPPH (IC<sub>50 </sub>80.64 ± 0.29 μg) and NO (IC<sub>50 </sub>4.12 ± 0.02 μg) assay. GC-MS analysis revealed the presence of over 38 compounds, the prevailing compounds were hexadecanoic acid, β-sitosterol, ethyl iso-allocholate and 9,12,15,-Octadecatrienoicacid. The HPLC analysis further confirmed the presence of rutin, salicylic acid and benzoic acid. This confirms that <em>T. indicum</em> could be the good source of natural antioxidant for industrial and pharmaceutical preparations.</p>
Highlights
Plants have remained as an excellent source of health promoting agents for mankind since thousands of years, and still today, they appear to have a huge share in the primary health care system
Almost all plants have some extent of antioxidant activity, even if, screening of plants for their antioxidant activity efficacy remains an interesting and useful field of research, especially for finding new natural antioxidant- entities, and/or to prove that one source of antioxidant is better in comparison with other [ 1,2]
The results of the preliminary phytochemical screening of hydromethanolic extract of T. indicum revealed the presence of steroids, phenolics, saponins, tannin and flavonoids (Table1)
Summary
Plants have remained as an excellent source of health promoting agents for mankind since thousands of years, and still today, they appear to have a huge share in the primary health care system. In vitro antioxidant assessment methods are often used to screen plants for their antioxidant properties. Almost all plants have some extent of antioxidant activity, even if, screening of plants for their antioxidant activity efficacy remains an interesting and useful field of research, especially for finding new natural antioxidant- entities, and/or to prove that one source of antioxidant is better in comparison with other [ 1,2]. And pharmacologicaliy, T. indicum is less studied plant species, though there are few reports on chemical constituents [8,9] and bioactivity [10,11,12,13,14,15,16,17]. We investigated the in-vitro antioxidant activity of the hydro-methanolic extract of aerial part of T. indicum using various models. The extract was chemically characterized to identify the active constituents
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
