Abstract
Aqueous size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups in the polysaccharide chains caused a polyelectrolyte expansion effect and neutral fractions of birch xylans, larch arabinogalactans (AG) and galactoglucomannans (GGM) were separated from neutral molecules in various concentrations of the injected samples
Highlights
Size Exclusion Chromatography often referred to as Gel Permeation Chromatography (GPC), is one of the most powerful methods for investigation and determination of molar-mass distribution (MMD) of polymers [1,2]
This paper aims to evaluate of charged anionic groups content of molecules in birch xylans, AG and GGM dissolved in water, as well as the influence of injected polymer concentrations to polyelectrolyte expansion effects in aqueous SEC
In separation of larch AG, multimodal chromatograms were found: the first peaks were appearing near the void volume of the column, indicating the presence of charged anionic groups in the molecules (Fig.1)
Summary
Size Exclusion Chromatography often referred to as Gel Permeation Chromatography (GPC), is one of the most powerful methods for investigation and determination of molar-mass distribution (MMD) of polymers [1,2]. This paper aims to evaluate of charged anionic groups content of molecules in birch xylans, AG and GGM dissolved in water, as well as the influence of injected polymer concentrations to polyelectrolyte expansion effects in aqueous SEC.
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