Abstract
Experimental infection was induced in immunosupreesed and non-immunosupressed mice using inoculation of two different Cryptosporidium spp. isolates: faecal origin and river water origin; then the infectivity rate and cellular immunological status were examined in these groups of mice. The results indicated that these two isolates induced experimental infection in immunosuppressed and non-immunosuppressed mice with no significant differences in infectivity rate between groups. Immunosuppressed mice infected with feacal or water origin oocyst showed low and significant values of transformed lymphocytes 8.68% ± 0.65%, 9.18% ± 1.09 respectively and phagocytic index 10.1% ± 1.66 and 11.2% ± 0.19 respectively. It concludes that lymphocyte transformation and phagocytosis index are good indicators to evaluate the cellular immune response in infected experimental mice with Cryptosporidium.
Highlights
IntroductionCryptosporidium spp. are eukaryotic protozoan of the Phylum Apicomplexa [1], more than twenty species of Cryptosporidium have been described from a variety of vertebrates (the parasite has been identified in greater than 150 vertebrate hosts) including mammals, birds, reptiles and fish [1,2,3,4]
Cryptosporidium spp. are eukaryotic protozoan of the Phylum Apicomplexa [1], more than twenty species of Cryptosporidium have been described from a variety of vertebrates including mammals, birds, reptiles and fish [1,2,3,4]
The aim of this study is to evaluate the cellular immune response using lymphocyte transformation and phagocytic index in mice infected with toqw isolates of Cryptosporidium
Summary
Cryptosporidium spp. are eukaryotic protozoan of the Phylum Apicomplexa [1], more than twenty species of Cryptosporidium have been described from a variety of vertebrates (the parasite has been identified in greater than 150 vertebrate hosts) including mammals, birds, reptiles and fish [1,2,3,4]. Whereas infections caused acute watery diarrhea only in neonatal ruminants and piglets, no symptoms are observed in most other infected animals of any age [7]. Because of these differences, different animal models are often required for different types of scientific studies. Materials and Methods Isolation of the Cryptosporidium spp Cryptosporidium spp was isolated from four high positive human faecal samples and two high positive water samples these samples were used for preparation the infected dose after concentration with Sheathers's sugar solution [8]: 1. Blood was taken from heart of infected and non infected mice using a sterile 1 ml syringe containing heparin
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