Evaluation of cellular antioxidant activity and chemical constituents of Melicope latifolia (DC.) T. G. Hartley

Abstract

Event Abstract Back to Event Evaluation of cellular antioxidant activity and chemical constituents of Melicope latifolia (DC.) T. G. Hartley Pei Cee Lim1, Nur Kartinee Kassim1*, Zulfiqar Ali2*, Ikhlas Khan2, 3, Shabana I. Khan2, 3, Khozirah Shaari1 and Amin Ismail4 1 Department of Chemistry, Faculty of Science, Putra Malaysia University, Malaysia 2 National Center for Natural Products Research, School of Pharmacy, University of Mississippi, United States 3 Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, United States 4 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia Background Melicope latifolia (DC.) T. G. Hartley, a Rutaceae family species, known as ‘kisampang’ and ‘pepau’ are distributed in Southeast Asia - Malaysia, Indonesia, Philippines to Papua New Guinea in the Pacific. Traditionally, Melicope latifolia leaves are used as remedy for fever and cramps. In Indonesia, the leaves are used in folk medicine to cure jaundice and malaria. Other Melicope species were reported to possess antiviral, anti-inflammatory and antioxidant activities. However, to date, there are limited reports on chemical constituents and biological potential of this species. Methods Powdered leaves of Melicope latifolia were extracted successively using hexanes, ethyl acetate and methanol. Total phenolic contents (TPC) and antioxidant capacities namely 2,2ʹ-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC) and copper reducing antioxidant capacity assay (CUPRAC) of the extracts were evaluated. Phytochemical study on the methanol extract yielded five flavonoids and flavonoid glycosides which were then subjected to cellular antioxidant activity (CAA) assay. Results Melicope latifolia methanol extract exhibited the highest phenolic content with 57mg GAE/g extract compared to other extracts. Maximum DPPH scavenging activity and ORAC values were also observed in methanol extract with 70mg TE/g extract and 1830µmol TE/g extract respectively. The methanol extract also possessed significant reducing ability on CUPRAC with the value of 187mg TE/g extract. Chemical constituents named taxifolin, quercetin 3-O-robinobioside, kaempferol 3-O-rutinoside, kaempferol 3-O-glucoside and kaempferol 3-O-arabinoside were isolated for the first time from this species. These compounds were subjected to CAA assay. Taxifolin was the most effective in decreasing the cellular oxidative stress compared to other compounds. Conclusion Methanol extract of Melicope latifolia appears to have the most significant antioxidant capacity. Isolated phenolic compounds from the extract certainly contribute to its anti-oxidative properties to some extent. Keywords: Rutaceae, Flavonoid, Melicope latifolia, Flavonoid glycosides, antioxidant Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Miscellaneous Citation: Lim P, Kassim N, Ali Z, Khan I, Khan SI, Shaari K and Ismail A (2019). Evaluation of cellular antioxidant activity and chemical constituents of Melicope latifolia (DC.) T. G. Hartley. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00086 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. 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Received: 18 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Nur Kartinee Kassim, Department of Chemistry, Faculty of Science, Putra Malaysia University, Serdang, Selangor, 43400, Malaysia, kartinee@upm.edu.my Dr. Zulfiqar Ali, National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi, 38677, United States, zulfiqar@olemiss.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Pei Cee Lim Nur Kartinee Kassim Zulfiqar Ali Ikhlas Khan Shabana I Khan Khozirah Shaari Amin Ismail Google Pei Cee Lim Nur Kartinee Kassim Zulfiqar Ali Ikhlas Khan Shabana I Khan Khozirah Shaari Amin Ismail Google Scholar Pei Cee Lim Nur Kartinee Kassim Zulfiqar Ali Ikhlas Khan Shabana I Khan Khozirah Shaari Amin Ismail PubMed Pei Cee Lim Nur Kartinee Kassim Zulfiqar Ali Ikhlas Khan Shabana I Khan Khozirah Shaari Amin Ismail Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.