Evaluation of cell wall-associated direct extracellular electron transfer in thermophilic Geobacillus sp.

Dummi Mahadevan Gurumurthy,Vaddi Damodara Reddy,Mohammed Azharuddin Savanur,Ganesh Dattatraya Saratale,Sikandar I Mulla,Muhammad Bilal,Luiz Fernando Romanholo Ferreira,Sanjay Kumar Gupta,Urszula Guzik,Ashok Kumar Nadda

doi:10.1007/s13205-021-02917-2

Abstract

The online version contains supplementary material available at 10.1007/s13205-021-02917-2.

Highlights

In bioelectrochemistry, the dissimilarity metal reduction by microbes is inter-related, as ubiquitous membrane proteins, conductive materials, and shuttling compounds are usually involved (Coursolle et al 2010; Fuller et al 2014; García-Angulo 2017; Kotloski and Gralnick 2013; von Canstein et al 2008; Wu et al 2016; Wu et al 2014)
In contrast to gram-positive strains (Carlson et al 2012; Dana JD 1997), the mechanism of electron transfer (EET) coupled with the metal reduction in gram-negative Shewanella (Brutinel and Gralnick 2012; Fernandes et al 2017; Gurav et al 2020; Hernandez and Newman 2001; Kotloski and Gralnick 2013; Lovley 1991; Myers and Myers 1992; Tokunou et al 2016; von Canstein et al 2008; White et al 2013) and Geobacter strains was widely studied (Cologgi et al 2011; Gurumurthy and Neelagund 2010; Hernandez and Newman 2001; Lovley 1991; Richter et al 2012; Zhao and Gurumurthy 2016)
EET pathway of gram-positive T. ferriacetica, it was proposed that the three multiheme c- cytochromes, Tfer_0070 (ImdcA), Tfer_0075 (CwcA), and Tfer_1887 (PdcA), are involved

Summary

Introduction

The dissimilarity metal reduction by microbes is inter-related, as ubiquitous membrane proteins, conductive materials, and shuttling compounds are usually involved (Coursolle et al 2010; Fuller et al 2014; García-Angulo 2017; Kotloski and Gralnick 2013; von Canstein et al 2008; Wu et al 2016; Wu et al 2014). Bioenergetics is usually accomplished by the transfer of its electrons(e-) from quinol moiety to a series of inner/outer membrane-bound multi-heme ctype cytochromes before it reaches to final electron acceptors (Fernandes et al 2017; Fuller et al 2014; Hernandez and Newman 2001; Zhao and Gurumurthy 2016; Zhao et al 2020) For such concept, bacteria usually develop two distinct mechanisms to deliver their electrons, i.e., (1) direct contact with metal oxides using membrane-bound c-type cytochromes (Mtr, OMCs) (Coursolle et al 2010; Hernandez and Newman 2001; Zhao and Gurumurthy 2016) and (2) by indirectly through soluble extracellular electron shuttles like flavins and humic substances (Brutinel and Gralnick 2012; Fuller et al 2014; Kotloski and Gralnick 2013; Lovley et al 1996; Wu et al 2016; Wu et al 2014). Microbially reduced uranium, Cr (III), and dimethyl sulfoxide (DMSO) are found at the cell surface and in the periplasm (Cologgi et al 2011)

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Results

Conclusion