Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens

Suellen Carvalho De Moura Braz,Yara De Miranda Gomes,Adriene Siqueira Melo,Maria Da Gloria Aureliano Melo Cavalcanti,Virginia Maria Barros De Lorena

doi:10.1590/0037-8682-1323-2013

Suellen Carvalho De Moura Braz, Yara De Miranda Gomes + Show 3 more

Open Access

https://doi.org/10.1590/0037-8682-1323-2013

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Abstract

CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures. Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease.

Highlights

CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease
This study aimed to evaluate detection of CD4+CD25+FoxP3+, CD4+CD25+interleukin 10 (IL10)+ T cells, and TGF-β in patients with chronic Chagas disease (ChD) at different cell culture time points after stimulation with CRA and FRA antigens
Comparison of the CARD and IND forms showed that the mean number of CD4+CD25+IL10+ T cells was higher in the IND group than in the CARD patients after stimulation with CRA and FRA, this difference was non-significant (Figures 1C and 1D)

Summary

Short Communication

Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens. Suellen Carvalho de Moura Braz[1], Virginia Maria Barros de Lorena[1],[2], Adriene Siqueira Melo[1], Maria da Glória Aureliano Melo Cavalcanti[3]. Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE. Programa Integrado de Doença de Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ. Pronto-socorro Cardiológico de Pernambuco, Universidade de Pernambuco, Recife, PE

CARD IND

IND CARD

Findings

FINANCIAL SUPPORT