Evaluation of Catalase and Manganese in Type 2 Diabetic Patients in University of Port Harcourt Teaching Hospital

Adaobi Maryann Ibekwe,Arvin Nwakulite,Bart I Ifionu,C C N Vincent,Calista Ndidi Adike,Chukwuma J Okafor,Ejike Felix Chukwurah,Emmanuel Ifeanyi Obeagu,Richard Eze,Ufuoma Chukwuani

doi:10.9734/jpri/2021/v33i30b31638

Abstract

This study was carried out to evaluate catalase and manganese in type 2 diabetes mellitus patients. A total of 40 subjects Were included in this study. This comprised of equal number of male and female subjects respectively. Enzyme linked immunosorbent assay (ELISA) method was used for this study. The result showed that there is a significant increase (P < 0.05) in the fasting blood sugar level of male diabetics (11.3±3.1) when compared with male non diabetics (4.0±0.4), and female diabetics (9.4±0.8) when compared with female non-diabetics (3.8±0.3). There is no significant increase (P<0.05) in the mean catalase value of male diabetics (77.3±137.7) when compared with male (256.3±207.6) and female non diabetics (225.1±233.9) But there is significant increase in female diabetics (27.8±14.7) when compared with controls (77.3±137.7) and female non diabetics (225.1±233.9).There is also significant decrease (P<0.05) in the mean Manganese value of male diabetics (0.5±0.6) when compared with male non diabetics (0.9±0.3) and female diabetic(0.2±0.1) when compared with female non-diabetics (0.3±0.1).The result shows that serum catalase level and manganese is decreased in diabetic patients. It is therefore suggested that type 2 diabetes patient should consume meals and supplement rich in catalase and manganese.

Highlights

Diabetes mellitus is characterized by absolute or relative deficiencies in insulin secretion and / or insulin action associated with chronic hyperglycemia and disturbances of carbohydrate, lipid and protein metabolism
The results showed that there is a significant increase (P < 0.05) in the fasting blood sugar (FBS) level of male diabetics (11.3±3.1) when compared with male non diabetics (4.0±0.4), and female diabetics (9.4±0.8) when compared with female non-diabetics (3.8±0.3).This correlates with Shobak et al [6] who states that type 2 diabetes mellitus is characterized by insulin resistance, which may be combined with relatively reduced insulin secretion
Serum catalase enzymes are decreased in type 2 diabetes subjects

Summary

Introduction

Diabetes mellitus is characterized by absolute or relative deficiencies in insulin secretion and / or insulin action associated with chronic hyperglycemia and disturbances of carbohydrate, lipid and protein metabolism. Long-term vascular complications represent a major cause of morbidity and mortality in patients with diabetes mellitus. Catalase is an enzyme commonly found in all living organism that require oxygen for existence (Bacteria, plant and animals). It is a very important enzyme in protecting the cell from oxidative damage by Reactive Oxygen Species (ROS). It is involved in any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression etc) as a result of a reactive oxygen species stimulus. The enzyme catalase, catalyzes the conversion of hydrogen peroxide to water and oxygen, using either an iron or manganese co-factor [2]

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