Abstract
Bone marrow from 14 dogs with proliferative disorders was evaluated by labeling cells with anti-CD45 and analyzing them by use of flow cytometry. Bone marrow from five healthy dogs was also evaluated to identify normal cell labeling patterns. The cells were displayed as the logarithm of red fluorescence intensity vs side-angle light scatter plots (CD45 scatter plots). For healthy dogs, the CD45 scatter plot technique identified four discrete cell populations. The immunophenotype of these cells identified these populations as granulocytes, erythroid cells, lymphocytes, and monocyte/macrophages. The CD45 scatter plot technique identified discrete populations of atypical cells in all dogs with proliferative disorders. Immunophenotyping of the atypical populations resulted in diagnoses of myelodysplastic syndrome, acute myeloid leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, metastatic lymphosarcoma, megakaryoblastic leukemia, and metastatic mast cell tumor. Possible clinical applications of the CD45 labeling technique include detection of malignant cell populations for immunophenotyping, early detection of metastasis of lymphosarcoma to bone marrow, detection of residual malignant cells after chemotherapy, and early detection of recurrence of leukemias.
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