Abstract
Evaluation of BrdU Influence on Sister Chromatid Exchange in Arctic and Silver Fox
Highlights
The sister chromatid exchange (SCE) test is a cytogenetic assay and is known as a biomonitoring test to analyse chromosome damage caused by exogenous factors
Significant differences were noted in the mean number of SCE between the analysed BrdU concentrations in the case of the arctic fox (P
Significant differences were found between the mean SCE values for the concentrations of 0.5 and 1.0 μg/ml vs. 2.5 μg/ml BrdU (P
Summary
The sister chromatid exchange (SCE) test is a cytogenetic assay and is known as a biomonitoring test to analyse chromosome damage caused by exogenous factors. The aim of the study was to analyse chromatin instability by determining the number and sites of spontaneous SCE in the karyotype of arctic and silver foxes. The experiment used peripheral blood lymphocytes following in vitro culture Karyotype evaluation in both fox species was made by RBA staining with modified Giemsa reagent. To analyse SCE, three different bromodeoxyuridine (BrdU) concentrations were added to the culture and the microscopic preparations were stained by the FPG technique. Based on these results, chromosomal polymorphism in the arctic fox and the modal number of B chromosomes in the silver fox were established. Spontaneous SCE in both fox species was observed at a concentration of 0.5 μg/ml BrdU. No significant effect of the living environment and no effect of sex on increasing SCE frequency were found in either fox species
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