Abstract
Background. Large mandibular defects are considered difficult reconstructive challenges for oral and maxillofacial surgeons. Cell therapy, as an alternative technique, might increase the speed of bone regeneration. This study aimed to investigate bone regeneration in large defects of dog mandibles using allogenic adipose-derived stem cells on gelatin foam as a cell carrier. Methods. The tissue engineering phase consisted of the sampling of adult dogs’ adipose tissue that can easily be isolated from adipose stem cells (ASCs) of the dogs, ASCs were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) with low glucose, containing 10% fetal bovine serum (FBS) (Sigma, USA) and 1% penicillin-streptomycin (Gibco, USA), with the characterization of dog ASCs and gelatin-transplanted ASCs. Six dogs were included in this experimental study in the next step and randomly assigned to the treatment and control groups. The samples in both groups underwent surgery under general anesthesia to create uniform 3-cm bony defects. The samples in both groups were reconstructed with titanium reconstruction plates and screws. A large bone gap filled with ASCs (5×106 ) was seeded on gelatin (ASCs) in the treatment group. In the control group, bony defects were filled with a cell delivery carrier without ASCs. Six months after transplantation, the animals’ mandibles were evaluated by CT scan imaging, and the results were quantified through the Hounsfield unit (HU). The data were analyzed with t-test. Results. Before transplantation, the nature of the stem cells was confirmed by the expression of CD44 and CD105 cell markers at 71.9% and 89.3%, respectively, and a lack of the CD45 cell marker expression at 2.2%. Evaluation of CT scan images showed significantly higher bone repair in the ASCs group (920.25±572.92 HU) than in the control group (-94.746± 08.42). Conclusion. The bone regeneration of the ASCs group was significantly higher than that in the control group.
Highlights
Large bone defects are a major clinical challenge due to delayed or incomplete bone healing
The obtained adipose tissue sample was washed with phosphatebuffered saline solution (PBS) containing 1% penicillinstreptomycin and chopped into 2–3-mm pieces with a scalpel blade and washed several times with PBS to remove excess tissues, such as blood cells and connective tissue
We studied the effect of adipose stem cells (ASCs) on bone regeneration without osteoinductive materials
Summary
Large bone defects are a major clinical challenge due to delayed or incomplete bone healing. If the bone defects go beyond the critical size defect, they will not heal without therapeutic intervention These critical size defects might be induced by trauma, infection, and tumor resection, and require bone grafts for reconstruction.[1]. This study aimed to investigate bone regeneration in large defects of dog mandibles using allogenic adipose-derived stem cells on gelatin foam as a cell carrier. Six dogs were included in this experimental study in the step and randomly assigned to the treatment and control groups The samples in both groups underwent surgery under general anesthesia to create uniform 3-cm bony defects. Bony defects were filled with a cell delivery carrier without ASCs. Six months after transplantation, the animals’ mandibles were evaluated by CT scan imaging, and the results were quantified through the Hounsfield unit (HU). The bone regeneration of the ASCs group was significantly higher than that in the control group
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