Evaluation of blood pressure measurement using a miniature blood pressure transmitter with jacketed external telemetry in cynomolgus monkeys

Abstract

IntroductionCurrent techniques used to accurately determine arterial blood pressure (BP) in conscious, unrestrained monkeys require invasive telemetry. This study evaluated the functionality of an implanted miniature telemetry blood pressure transmitter for the collection of BP measurements in conjunction with electrocardiographic measurements using a jacketed external telemetry (JET™) system in conscious, unrestrained cynomolgus monkeys. MethodsTwenty-four animals were surgically implanted with the transmitter in the right femoral artery. Local tolerability to the implant, signal quality, and variability in hemodynamic values were evaluated. On alternate weeks, animals were given single doses of positive control agents to produce hypotensive (clonidine hydrochloride) or hypertensive (L-NAME) effects. Undisturbed telemetry BP data were continuously collected for at least 24h following dosing and analyzed. ResultsWhile exhibiting remarkably high signal quality (∼95% data points retained over 24h of data collection) and moderate variability across study weeks in baseline pulse height measurements (changes as small as <0mmHg), nine of 18 transmitters were nonfunctional by 19weeks post-surgery, most likely due to migration of the catheter out of the artery. In animals given positive control agents, L-NAME induced a statistically significant increase (≥+8mmHg) and clonidine hydrochloride induced a statistically significant decrease (−11mmHg) in mean arterial pressures. Histological analysis revealed femoral arterial thickening near the sites of implantation. DiscussionThese results demonstrate the ability of the miniature BP transmitter, in conjunction with the JET™ system, to detect small changes in hemodynamic data continuously collected in conscious unrestrained monkeys. Future optimization of the transmitter includes the addition of a suture rib to the transmitter body and increased catheter size to prevent catheter migration out of the artery, the root cause of failed transmitters. The miniature blood pressure transmitter evaluated provides a minimally invasive technique for continuous collection of hemodynamic data in a toxicology study environment.