EVALUATION OF BISPHENOL A INDUCED EFFECTS ON BLOOD BIO-CHEMICAL CONSTITUENTS AND HISTO-STRUCTURE OF LIVER IN SWISS ALBINO MICE AND ITS ‘ONE HEALTH’ PERSPECTIVES

Abstract

Background: Bisphenol A (BPA) is a plastic synthetic chemical ingredient produced widely in large quantities for use primarily in production of polycarbonate plastics and epoxy resins worldwide. The toxicity of BPA in human health is associated with enzymatic, androgenic, neurologic, liver and reproductive systems which have been reported but its toxicokinetics has not been established and even its status is not documented in Bangladesh. Objectives: The aim of this study was to detect the BPA induced effects on blood bio-chemical constituents and histo-texture of liver in adult Swiss albino mice. Materials and Methods: Fifteen mice, 6 to 8 weeks of age with an average bwt 27.1  0.5g, were equally divided into three groups (n = 5). Group A (control) received only normal mouse pellet feed while groups B and C received pellet feed mixed with BPA (Sigma-Aldrich Co., USA) @ 50 mg and 100 mg / kg bwt daily for 12 weeks, respectively. Sera of all the mice were tested for biochemical constituents and liver tissues for histo-pathological studies. Results: Results showed that cholesterol and LDL levels were elevated significantly (p < 0.01) in both the BPA treated groups (B and C) respectively in comparison to control group A. The HDL cholesterol and TG levels in mice of group C treated with BPA @100 mg / kg BW were significantly (p < 0.01) reduced in comparison to control group of mice. Serum glucose level was significantly (p < 0.1) decreased in both the BPA treated groups (B and C) whereas total serum protein level in mice of group C significantly (p < 0.1) increased in comparison to control group A. The liver enzymes (ALT, AST & ALP) were also significantly (p < 0.01) increased in BPA treated mice in comparison to control. Histo-pathological alterations were also detected in the liver of BPA-treated mice of both the groups. Conclusions: These findings provide evidence of changes in the blood bio-chemical constituents and liver histo-texture induced by BPA and may have implications for understanding the toxicity of BPA in animals and humans. Further research may be performed on the status of level of BPA in food and beverage of plastic containers and their impact on human health in Bangladesh. Keywords: Bisphenol A, Mice, Induced effects, Biochemical constituents, Histo-structure of liver, Public health importance