Abstract
ABSTRACTThis paper compared the results of osteoblast-like UMR-106 cells response to 3-D biodegradable porous with nonporous PHBV (poly (3-hydroxybutyrate-co-3-hydroxyvalerate) films. Nonporous PHBV films were prepared by solvent casting and evaporation. Porous PHBV films were prepared by solute leaching of salt/PHBV cast film. Sieved sodium chloride (∼150 μm) was used to create a matrix with high porosity and then leached out by the solvent. Thermo gravimetric analysis showed that over 99% of the salts were leached out by the solvent. Osteoblast-like UMR-106 cells were seeded onto the nonporous and porous PHBV films, respectively. After 6 and 10 days of incubation, growth rate of UMR-106 cells on porous film was higher than on nonporous film. The viable cell proliferation on the porous and nonporous PHBV film was quantified by (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay. The results of this study suggest that the UMR-106 cells proliferate more on the porous PHBV matrix and porous PHBV films are a promising material for bone tissue engineering applications.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
