Evaluation of biofilm formation on acrylic resin surfaces coated with silicon dioxide: an in situ study.

Rossano Vinícius Dala Rosa Silva,Renata Corrêa Pascotto,Sharanbir Kaur Sidhu,Isabele Carrilho Jarros,Altair Antoninha Del Bel Cury,Sónia Silva,Melyssa Fernanda Norman Negri

doi:10.1590/1807-3107bor-2022.vol36.0007

Rossano Vinícius Dala Rosa Silva, Renata Corrêa Pascotto + Show 5 more

Open Access

https://doi.org/10.1590/1807-3107bor-2022.vol36.0007

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Abstract

Biofilm on acrylic resin dental prostheses may cause gingival inflammation. This study evaluated the influence of a silicon dioxide coating layer applied onto acrylic resin on the adhesion of microorganisms. Blocks (5 x 5 x 3 mm) of acrylic resin were evaluated for surface roughness and divided into two groups: control (CG) and coated with silicon dioxide (LG group). The specimens were evaluated by scanning electron microscopy (n = 1) and by contact angle analysis (n = 3). For the in situ study, 20 volunteers wore acrylic palatal devices containing three samples from each group (n = 60) for 2 days. The biofilm formed was quantified by metabolic activity and total biomass using the crystal violet assay. The results were subjected to Bartlett's normality test and Gamma model with random effect for the response variable (α = 5%). The mean contact angle of the coated group was significantly lower than that of the uncoated group (p < 0.05). The metabolic activity of microorganisms in the biofilm on the blocks treated with coating was significantly lower than that of control blocks (p = 0.02). Regarding the amount of extracellular matrix produced by the microorganisms, there was no difference between the CG and LG group (p = 0.05). The application of a silicon dioxide coating on acrylic resin reduced the activity of the polymicrobial biofilm formed in situ. This coating may be advantageous for patients with conventional complete dentures or implants made of acrylic resin and who have motor difficulties that prevent them from cleaning their prostheses properly.

Highlights

In the last National Oral Health Survey[1] in Brazil carried out in 2010, despite a significant decrease in the rate of dental caries in different age groups and an increase in access to health services, the population continues to lose teeth throughout life[1]
Regarding the ability to donate and receive electrons (γ- and γ +, there was a change in polarity, in which the coated group (CG) had lower γ + (12.0) and greater γ - (14.4), whereas the LG group presented greater γ + (2.1) and lower γ- (1.6), changing the surface from polar to non-polar
The metabolic activity of microorganisms in the biofilm formed on the blocks treated with silicon dioxide (LG group) was significantly lower than on the control (CG) blocks (p = 0.02)

Summary

Introduction

In the last National Oral Health Survey[1] in Brazil carried out in 2010, despite a significant decrease in the rate of dental caries in different age groups and an increase in access to health services, the population continues to lose teeth throughout life[1].

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Conclusion