Evaluation of biocidal activity of Evolyse, a disinfectant based on hydrogen peroxide and silver nitrate

Abstract

The in vitro biocidal activity of a novel disinfectant, Evolyse, against a wide spectrum of microorganisms, including bacteria, spores and fungi was analysed. The antimicrobial efficacy of disinfectant delivered by a nebulising device was also evaluated under practical conditions, in different hospital environments by microbiological monitoring of air and surfaces, before and after disinfection treatment. The disinfectant exhibited bactericidal activity against all bacterial strains tested after 5 min contact at room temperature. Sporicidal activity against B. cereus ATCC 11178 spores was obtained after 45 min contact at room temperature. The disinfectant also showed fungicidal activity against A. flavus ATCC 46283 and an A. niger DBS strain after 15 min contact at room temperature and against C. albicans ATCC 10231, S. cerevisiae ATCC 2601 and P. chrysogenum ATCC 9179 after 30 min contact time. Overall, disinfection treatment with Evolyse was able to reduce up to 70% of the microbial growth of air and surfaces of the different hospital environments analysed. The results of this study showed the good biocidal activity of Evolyse and offer promising perspective for the use of this product in hospital environments.